A skin sensitiser refers to a substance that will lead to an allergic response following repeated skin contact.
Sensitization testing is used to assess the potential of test substance to cause hypersensitivity reaction following exposure.

• Skin Sensitization-Local Lymph Node Assay : BrdU-ELISA

  Guidelines

  • OECD Guideline 442B(Skin Sensitization : Local Lymph Node Assay: BrdU-ELISA)
  • 국립환경과학원 고시 화학물질의 시험항법에 관한 규정; 제5장 건강영향 시험분야, 제35항 피부과민성시험(국소림프절시험, LLNA: BrdU-ELISA)
  • 농촌진흥청 고시 농약 및 원제의 등록기준; [별표12] 인축 독성 시험기준과 방법, 12-1-23. 피부감작성시험: 국소림프절시험법-BrdU-ELISA(Local Lymph Node Assay- BrdU-ELISA)

• Receipt of animal

• Acclimation

• Group assignment

• Administration

• Observation

• Excise the
  lymph nodes

• absorbance
  measure